MitoPlantRedoxV1, Main, Exploration, bibRecord, 000015

Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.

Identifieur interne : 000015 ( Main/Exploration ); précédent : 000014; suivant : 000016

Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.

Auteurs : Thomas Nietzel [Allemagne] ; Jörg Mostertz [Allemagne] ; Cristina Ruberti [Allemagne] ; Guillaume Née [Allemagne] ; Philippe Fuchs [Allemagne] ; Stephan Wagner [Allemagne] ; Anna Moseler [Allemagne, France] ; Stefanie J. Müller-Schüssele [Allemagne] ; Abdelilah Benamar [France] ; Gernot Poschet [Allemagne] ; Michael Büttner [Allemagne] ; Ian Max M Ller [Danemark] ; Christopher H. Lillig [Allemagne] ; David Macherel [France] ; Markus Wirtz [Allemagne] ; Rüdiger Hell [Allemagne] ; Iris Finkemeier [Allemagne] ; Andreas J. Meyer [Allemagne] ; Falko Hochgr Fe [Allemagne] ; Markus Schwarzl Nder [Allemagne]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.

RBID : pubmed:31871212

Descripteurs français

KwdFr :
- Adénosine triphosphate (métabolisme), Arabidopsis (physiologie), Cycle citrique (physiologie), Germination (physiologie), Glutathione reductase (génétique), Glutathione reductase (métabolisme), Graines (croissance et développement), Graines (cytologie), Graines (métabolisme), Mitochondries (métabolisme), Oxydoréduction (MeSH), Oxygène (métabolisme), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Protéomique (méthodes), Thioredoxin-disulfide reductase (génétique), Thioredoxin-disulfide reductase (métabolisme), Thiorédoxine h (génétique), Thiorédoxine h (métabolisme), Végétaux génétiquement modifiés (MeSH).
MESH :
- croissance et développement : Graines.
- cytologie : Graines.
- génétique : Glutathione reductase, Protéines d'Arabidopsis, Thioredoxin-disulfide reductase, Thiorédoxine h.
- métabolisme : Adénosine triphosphate, Glutathione reductase, Graines, Mitochondries, Oxygène, Protéines d'Arabidopsis, Thioredoxin-disulfide reductase, Thiorédoxine h.
- méthodes : Protéomique.
- physiologie : Arabidopsis, Cycle citrique, Germination.
- Oxydoréduction, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Adenosine Triphosphate (metabolism), Arabidopsis (physiology), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Citric Acid Cycle (physiology), Germination (physiology), Glutathione Reductase (genetics), Glutathione Reductase (metabolism), Mitochondria (metabolism), Oxidation-Reduction (MeSH), Oxygen (metabolism), Plants, Genetically Modified (MeSH), Proteomics (methods), Seeds (cytology), Seeds (growth & development), Seeds (metabolism), Thioredoxin h (genetics), Thioredoxin h (metabolism), Thioredoxin-Disulfide Reductase (genetics), Thioredoxin-Disulfide Reductase (metabolism).
MESH :
- chemical , genetics : Arabidopsis Proteins, Glutathione Reductase, Thioredoxin h, Thioredoxin-Disulfide Reductase.
- chemical , metabolism : Adenosine Triphosphate, Arabidopsis Proteins, Glutathione Reductase, Oxygen, Thioredoxin h, Thioredoxin-Disulfide Reductase.
- cytology : Seeds.
- growth & development : Seeds.
- metabolism : Mitochondria, Seeds.
- methods : Proteomics.
- physiology : Arabidopsis, Citric Acid Cycle, Germination.
- Oxidation-Reduction, Plants, Genetically Modified.

Abstract

Seeds preserve a far developed plant embryo in a quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since the switchover from quiescence to reactivation provides a remarkable case of a cell physiological transition we investigated the earliest events in energy and redox metabolism of Arabidopsis seeds at imbibition. By developing fluorescent protein biosensing in intact seeds, we observed ATP accumulation and oxygen uptake within minutes, indicating rapid activation of mitochondrial respiration, which coincided with a sharp transition from an oxidizing to a more reducing thiol redox environment in the mitochondrial matrix. To identify individual operational protein thiol switches, we captured the fast release of metabolic quiescence in organello and devised quantitative iodoacetyl tandem mass tag (iodoTMT)-based thiol redox proteomics. The redox state across all Cys peptides was shifted toward reduction from 27.1% down to 13.0% oxidized thiol. A large number of Cys peptides (412) were redox switched, representing central pathways of mitochondrial energy metabolism, including the respiratory chain and each enzymatic step of the tricarboxylic acid (TCA) cycle. Active site Cys peptides of glutathione reductase 2, NADPH-thioredoxin reductase a/b, and thioredoxin-o1 showed the strongest responses. Germination of seeds lacking those redox proteins was associated with markedly enhanced respiration and deregulated TCA cycle dynamics suggesting decreased resource efficiency of energy metabolism. Germination in aged seeds was strongly impaired. We identify a global operation of thiol redox switches that is required for optimal usage of energy stores by the mitochondria to drive efficient germination.

DOI: 10.1073/pnas.1910501117
PubMed: 31871212
PubMed Central: PMC6955286

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000049
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Le document en format XML

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<author><name sortKey="Macherel, David" sort="Macherel, David" uniqKey="Macherel D" first="David" last="Macherel">David Macherel</name>
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<author><name sortKey="Finkemeier, Iris" sort="Finkemeier, Iris" uniqKey="Finkemeier I" first="Iris" last="Finkemeier">Iris Finkemeier</name>
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<author><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name>
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<author><name sortKey="Hochgr Fe, Falko" sort="Hochgr Fe, Falko" uniqKey="Hochgr Fe F" first="Falko" last="Hochgr Fe">Falko Hochgr Fe</name>
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<author><name sortKey="Schwarzl Nder, Markus" sort="Schwarzl Nder, Markus" uniqKey="Schwarzl Nder M" first="Markus" last="Schwarzl Nder">Markus Schwarzl Nder</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.</title>
<author><name sortKey="Nietzel, Thomas" sort="Nietzel, Thomas" uniqKey="Nietzel T" first="Thomas" last="Nietzel">Thomas Nietzel</name>
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<author><name sortKey="Mostertz, Jorg" sort="Mostertz, Jorg" uniqKey="Mostertz J" first="Jörg" last="Mostertz">Jörg Mostertz</name>
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<author><name sortKey="Ruberti, Cristina" sort="Ruberti, Cristina" uniqKey="Ruberti C" first="Cristina" last="Ruberti">Cristina Ruberti</name>
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<author><name sortKey="Nee, Guillaume" sort="Nee, Guillaume" uniqKey="Nee G" first="Guillaume" last="Née">Guillaume Née</name>
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<country xml:lang="fr">Allemagne</country>
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<region type="district" nuts="2">District de Münster</region>
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<author><name sortKey="Fuchs, Philippe" sort="Fuchs, Philippe" uniqKey="Fuchs P" first="Philippe" last="Fuchs">Philippe Fuchs</name>
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<affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany.</nlm:affiliation>
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<author><name sortKey="Wagner, Stephan" sort="Wagner, Stephan" uniqKey="Wagner S" first="Stephan" last="Wagner">Stephan Wagner</name>
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<wicri:regionArea>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster</wicri:regionArea>
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<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
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<affiliation wicri:level="3"><nlm:affiliation>Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max Planck Institute for Plant Breeding Research, D-50829 Cologne</wicri:regionArea>
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<author><name sortKey="Moseler, Anna" sort="Moseler, Anna" uniqKey="Moseler A" first="Anna" last="Moseler">Anna Moseler</name>
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<region type="district" nuts="2">District de Cologne</region>
<settlement type="city">Bonn</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:affiliation>Faculté des Sciences et Technologies, UMR 1136 Interactions Arbres/Microorganismes, Université de Lorraine, F-54506 Vandœuvre-lès-Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
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<placeName><region type="region" nuts="2">Grand Est</region>
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<author><name sortKey="Muller Schussele, Stefanie J" sort="Muller Schussele, Stefanie J" uniqKey="Muller Schussele S" first="Stefanie J" last="Müller-Schüssele">Stefanie J. Müller-Schüssele</name>
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<author><name sortKey="Benamar, Abdelilah" sort="Benamar, Abdelilah" uniqKey="Benamar A" first="Abdelilah" last="Benamar">Abdelilah Benamar</name>
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<country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Recherche en Horticulture et Semences, Université d'Angers, UMR 1345, F-49071 Beaucouzé Cedex</wicri:regionArea>
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<settlement type="city">Beaucouzé</settlement>
</placeName>
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</affiliation>
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<author><name sortKey="Poschet, Gernot" sort="Poschet, Gernot" uniqKey="Poschet G" first="Gernot" last="Poschet">Gernot Poschet</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Buttner, Michael" sort="Buttner, Michael" uniqKey="Buttner M" first="Michael" last="Büttner">Michael Büttner</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="M Ller, Ian Max" sort="M Ller, Ian Max" uniqKey="M Ller I" first="Ian Max" last="M Ller">Ian Max M Ller</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Genetics, Aarhus University, DK-4200 Slagelse, Denmark.</nlm:affiliation>
<country xml:lang="fr">Danemark</country>
<wicri:regionArea>Department of Molecular Biology and Genetics, Aarhus University, DK-4200 Slagelse</wicri:regionArea>
<wicri:noRegion>DK-4200 Slagelse</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lillig, Christopher H" sort="Lillig, Christopher H" uniqKey="Lillig C" first="Christopher H" last="Lillig">Christopher H. Lillig</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute for Medical Biochemistry and Molecular Biology, University Medicine Greifswald, D-17489 Greifswald, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute for Medical Biochemistry and Molecular Biology, University Medicine Greifswald, D-17489 Greifswald</wicri:regionArea>
<wicri:noRegion>17489 Greifswald</wicri:noRegion>
<wicri:noRegion>17489 Greifswald</wicri:noRegion>
<wicri:noRegion>D-17489 Greifswald</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Macherel, David" sort="Macherel, David" uniqKey="Macherel D" first="David" last="Macherel">David Macherel</name>
<affiliation wicri:level="4"><nlm:affiliation>Institut de Recherche en Horticulture et Semences, Université d'Angers, UMR 1345, F-49071 Beaucouzé Cedex, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Recherche en Horticulture et Semences, Université d'Angers, UMR 1345, F-49071 Beaucouzé Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Beaucouzé</settlement>
</placeName>
<orgName type="university">Université d'Angers</orgName>
</affiliation>
</author>
<author><name sortKey="Wirtz, Markus" sort="Wirtz, Markus" uniqKey="Wirtz M" first="Markus" last="Wirtz">Markus Wirtz</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hell, Rudiger" sort="Hell, Rudiger" uniqKey="Hell R" first="Rüdiger" last="Hell">Rüdiger Hell</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Centre for Organismal Studies Heidelberg, Heidelberg University, D-69120 Heidelberg</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Heidelberg</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Finkemeier, Iris" sort="Finkemeier, Iris" uniqKey="Finkemeier I" first="Iris" last="Finkemeier">Iris Finkemeier</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Cologne</region>
<settlement type="city">Bonn</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hochgr Fe, Falko" sort="Hochgr Fe, Falko" uniqKey="Hochgr Fe F" first="Falko" last="Hochgr Fe">Falko Hochgr Fe</name>
<affiliation wicri:level="1"><nlm:affiliation>Competence Center Functional Genomics, University of Greifswald, D-17489 Greifswald, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Competence Center Functional Genomics, University of Greifswald, D-17489 Greifswald</wicri:regionArea>
<wicri:noRegion>17489 Greifswald</wicri:noRegion>
<wicri:noRegion>17489 Greifswald</wicri:noRegion>
<wicri:noRegion>D-17489 Greifswald</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schwarzl Nder, Markus" sort="Schwarzl Nder, Markus" uniqKey="Schwarzl Nder M" first="Markus" last="Schwarzl Nder">Markus Schwarzl Nder</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany; markus.schwarzlander@uni-muenster.de.</nlm:affiliation>
<country wicri:rule="url">Allemagne</country>
<wicri:regionArea>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (metabolism)</term>
<term>Arabidopsis (physiology)</term>
<term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
<term>Citric Acid Cycle (physiology)</term>
<term>Germination (physiology)</term>
<term>Glutathione Reductase (genetics)</term>
<term>Glutathione Reductase (metabolism)</term>
<term>Mitochondria (metabolism)</term>
<term>Oxidation-Reduction (MeSH)</term>
<term>Oxygen (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Proteomics (methods)</term>
<term>Seeds (cytology)</term>
<term>Seeds (growth & development)</term>
<term>Seeds (metabolism)</term>
<term>Thioredoxin h (genetics)</term>
<term>Thioredoxin h (metabolism)</term>
<term>Thioredoxin-Disulfide Reductase (genetics)</term>
<term>Thioredoxin-Disulfide Reductase (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate (métabolisme)</term>
<term>Arabidopsis (physiologie)</term>
<term>Cycle citrique (physiologie)</term>
<term>Germination (physiologie)</term>
<term>Glutathione reductase (génétique)</term>
<term>Glutathione reductase (métabolisme)</term>
<term>Graines (croissance et développement)</term>
<term>Graines (cytologie)</term>
<term>Graines (métabolisme)</term>
<term>Mitochondries (métabolisme)</term>
<term>Oxydoréduction (MeSH)</term>
<term>Oxygène (métabolisme)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Protéines d'Arabidopsis (métabolisme)</term>
<term>Protéomique (méthodes)</term>
<term>Thioredoxin-disulfide reductase (génétique)</term>
<term>Thioredoxin-disulfide reductase (métabolisme)</term>
<term>Thiorédoxine h (génétique)</term>
<term>Thiorédoxine h (métabolisme)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term>
<term>Glutathione Reductase</term>
<term>Thioredoxin h</term>
<term>Thioredoxin-Disulfide Reductase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphate</term>
<term>Arabidopsis Proteins</term>
<term>Glutathione Reductase</term>
<term>Oxygen</term>
<term>Thioredoxin h</term>
<term>Thioredoxin-Disulfide Reductase</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Graines</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Graines</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutathione reductase</term>
<term>Protéines d'Arabidopsis</term>
<term>Thioredoxin-disulfide reductase</term>
<term>Thiorédoxine h</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mitochondria</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénosine triphosphate</term>
<term>Glutathione reductase</term>
<term>Graines</term>
<term>Mitochondries</term>
<term>Oxygène</term>
<term>Protéines d'Arabidopsis</term>
<term>Thioredoxin-disulfide reductase</term>
<term>Thiorédoxine h</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Arabidopsis</term>
<term>Cycle citrique</term>
<term>Germination</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Arabidopsis</term>
<term>Citric Acid Cycle</term>
<term>Germination</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Oxidation-Reduction</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Oxydoréduction</term>
<term>Végétaux génétiquement modifiés</term>
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<front><div type="abstract" xml:lang="en">Seeds preserve a far developed plant embryo in a quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since the switchover from quiescence to reactivation provides a remarkable case of a cell physiological transition we investigated the earliest events in energy and redox metabolism of <i>Arabidopsis</i>
 seeds at imbibition. By developing fluorescent protein biosensing in intact seeds, we observed ATP accumulation and oxygen uptake within minutes, indicating rapid activation of mitochondrial respiration, which coincided with a sharp transition from an oxidizing to a more reducing thiol redox environment in the mitochondrial matrix. To identify individual operational protein thiol switches, we captured the fast release of metabolic quiescence in organello and devised quantitative iodoacetyl tandem mass tag (iodoTMT)-based thiol redox proteomics. The redox state across all Cys peptides was shifted toward reduction from 27.1% down to 13.0% oxidized thiol. A large number of Cys peptides (412) were redox switched, representing central pathways of mitochondrial energy metabolism, including the respiratory chain and each enzymatic step of the tricarboxylic acid (TCA) cycle. Active site Cys peptides of glutathione reductase 2, NADPH-thioredoxin reductase a/b, and thioredoxin-o1 showed the strongest responses. Germination of seeds lacking those redox proteins was associated with markedly enhanced respiration and deregulated TCA cycle dynamics suggesting decreased resource efficiency of energy metabolism. Germination in aged seeds was strongly impaired. We identify a global operation of thiol redox switches that is required for optimal usage of energy stores by the mitochondria to drive efficient germination.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31871212</PMID>
<DateCompleted><Year>2020</Year>
<Month>05</Month>
<Day>05</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>06</Month>
<Day>23</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>117</Volume>
<Issue>1</Issue>
<PubDate><Year>2020</Year>
<Month>01</Month>
<Day>07</Day>
</PubDate>
</JournalIssue>
<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
<ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation>
</Journal>
<ArticleTitle>Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.</ArticleTitle>
<Pagination><MedlinePgn>741-751</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1910501117</ELocationID>
<Abstract><AbstractText>Seeds preserve a far developed plant embryo in a quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since the switchover from quiescence to reactivation provides a remarkable case of a cell physiological transition we investigated the earliest events in energy and redox metabolism of <i>Arabidopsis</i>
 seeds at imbibition. By developing fluorescent protein biosensing in intact seeds, we observed ATP accumulation and oxygen uptake within minutes, indicating rapid activation of mitochondrial respiration, which coincided with a sharp transition from an oxidizing to a more reducing thiol redox environment in the mitochondrial matrix. To identify individual operational protein thiol switches, we captured the fast release of metabolic quiescence in organello and devised quantitative iodoacetyl tandem mass tag (iodoTMT)-based thiol redox proteomics. The redox state across all Cys peptides was shifted toward reduction from 27.1% down to 13.0% oxidized thiol. A large number of Cys peptides (412) were redox switched, representing central pathways of mitochondrial energy metabolism, including the respiratory chain and each enzymatic step of the tricarboxylic acid (TCA) cycle. Active site Cys peptides of glutathione reductase 2, NADPH-thioredoxin reductase a/b, and thioredoxin-o1 showed the strongest responses. Germination of seeds lacking those redox proteins was associated with markedly enhanced respiration and deregulated TCA cycle dynamics suggesting decreased resource efficiency of energy metabolism. Germination in aged seeds was strongly impaired. We identify a global operation of thiol redox switches that is required for optimal usage of energy stores by the mitochondria to drive efficient germination.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nietzel</LastName>
<ForeName>Thomas</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mostertz</LastName>
<ForeName>Jörg</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Competence Center Functional Genomics, University of Greifswald, D-17489 Greifswald, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ruberti</LastName>
<ForeName>Cristina</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Née</LastName>
<ForeName>Guillaume</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fuchs</LastName>
<ForeName>Philippe</ForeName>
<Initials>P</Initials>
<Identifier Source="ORCID">0000-0001-6379-853X</Identifier>
<AffiliationInfo><Affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wagner</LastName>
<ForeName>Stephan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Institute of Plant Biology and Biotechnology, University of Münster, D-48143 Münster, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Moseler</LastName>
<ForeName>Anna</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Faculté des Sciences et Technologies, UMR 1136 Interactions Arbres/Microorganismes, Université de Lorraine, F-54506 Vandœuvre-lès-Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Müller-Schüssele</LastName>
<ForeName>Stefanie J</ForeName>
<Initials>SJ</Initials>
<Identifier Source="ORCID">0000-0003-4061-1175</Identifier>
<AffiliationInfo><Affiliation>Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Benamar</LastName>
<ForeName>Abdelilah</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institut de Recherche en Horticulture et Semences, Université d'Angers, UMR 1345, F-49071 Beaucouzé Cedex, France.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Poschet</LastName>
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<name sortKey="Macherel, David" sort="Macherel, David" uniqKey="Macherel D" first="David" last="Macherel">David Macherel</name>
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<country name="Danemark"><noRegion><name sortKey="M Ller, Ian Max" sort="M Ller, Ian Max" uniqKey="M Ller I" first="Ian Max" last="M Ller">Ian Max M Ller</name>
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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000015 | SxmlIndent | more

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{{Explor lien
   |wiki=    Bois
   |area=    MitoPlantRedoxV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:31871212
   |texte=   Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.
}}

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	Serveur d'exploration sur les mitochondries dans l'oxydoréduction chez les plantes
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les mitochondries dans l'oxydoréduction chez les plantes

Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.

Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination.

Source :

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